详细描述:
RNAok™ 核糖核酸酶抑制剂是由E. coli表达并亲和层析纯化的重组的哺乳动物核糖核酸酶抑制剂,通过强烈的1:1的非竞争性结合,抑制胰型核醣核酸、 RNaseA、 B 和 C。RNAok™ 核糖核酸酶抑制剂不能抑制真核生物的核酸酶 T1,T2,U1、 U2、 CL3,以及原核核糖核酸I and H。可与逆转录酶例如AMV,M-MLV 和ExcelRT™ R逆转录酶或Taq DNA聚合酶配合使用。RNAok™ RNase Inhibitor is a recombinant mammalian RNase inhibitor that is purified by affinity chromatography from E. coli. This protein inhibits pancreatic-type ribonucleases, RNaseA, B, and C by binding strongly to RNases in a noncompetitive mode at a 1:1 ratio. RNAok™RNase Inhibitor does not inhibit eukaryotic RNases T1, T2, U1, U2, CL3 as well as prokaryotic RNases I and H. RNAok™ RNase Inhibitor is compatible with RT-PCR enzymes such as AMV,M-MLV and ExcelRT™ Reverse Transcriptase or Taq DNA polymerase. Extensive quality control tests ensure RNAok™ RNase Inhibitor is free of unwanted contaminants that can plague other commercially available preparations of RNase inhibitors.