详细描述:
ExcelRT一步法RT-PCR试剂盒 (ExcelRT One-Step RT-PCR Kit, RP1100),可在同一个反应管内一步完成逆转录与PCR反应实验,利用RNA作为模板以目标基因的正向和反向引物合成cDNA及进行PCR反应。 本产品为两倍预混(Premix)反应试剂,内含优化的缓冲液、dNTPs、Mg2+、stabilizer,及预混的逆转录酶与常规型DNA Taq酶,提供用户一个便捷的实验方式,且本产品仅需于PCR聚合酶反应器 (thermal cycler) 内设定一个程序依序进行逆转录与PCR反应,即便是微量的RNA也可被检测出来。The ExcelRTTM One-Step RT-PCR Kit is designed for the reverse transcription and PCR amplification of a specific target gene from either total RNA or mRNA. The ExcelRTTM One-Step RT-PCR Kit provides users an alternative to two lengthy steps (first strand generation and amplification) by using a single mixture in a single tube for the one-step reaction. The ExcelRTTM One-Step RT-PCR Kit contains a 2X reaction premix consisting of an optimized buffer, dNTPs, Mg2+, enzyme stabilizer, and a blend of recombinant reverse transcriptase and Taq DNA polymerase. The ExcelRTTM One-Step RT-PCR Kit allows users to complete the RT-PCR process using a thermocycler in a single reaction setting and is ideal for target RNA amplification. This product is capable of detecting even trace amounts of target RNA.