详细描述:
ExcelRT™ 反转录试剂盒是一个完整、 高效、 简便的试剂盒,可合成高质量的条互补DNA链 (cDNA)。试剂盒包含可在37~50°C稳定合成条互补DNA链 (cDNA)的逆转录酶。从莫罗尼鼠白血病病毒(Moloney murine leukemin virus)的逆转录酶(简称M-MLV)重组改良而来,降低了核糖核酸酶H (RNase H)活性,增加其热稳定性。试剂盒还包括RNAok™ Rnase抑制剂,可以有效抵抗 RNase A, RNase B, and RNase C。同时提供 oligo (dT)20和随机引物,分别用来合成以 poly(A)结尾的mRNA 和 Totoal RNA的cDNA链。ExcelRT™ Reverse Transcription Kit is a complete, efficient and convenient kit to synthesize high quality first strand cDNA. This kit contains ExcelRT™ Reverse Transcriptase, which is able to synthesize the first strand cDNA at 37~50°C. The ExcelRT™ Reverse Transcriptase is a recombinant Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase, which is designed to reduce RNase H activity and create better thermal stability. This kit also contains RNAok™ RNase Inhibitor, which is active against RNase A, RNase B, and RNase C. This product is supplied with oligo (dT)20 and random hexamers, which are used to synthesize cDNA from poly(A) tailed mRNA and total RNA, respectively.