ExcelTaq 5X PCR 预混试剂盒 (ExcelTaq 5X PCR Master Mix, TP1100) 为一组直接使用于扩增目标DNA片段的产品。 此产品设计的目的为提供给有PCR应用需求的用户可立即使用,不需额外准备其他试剂。 这组PCR反应预混液内含大部分PCR实验所需之成分,用户只需另外加入模板和特异性引物。 本产品节省许多操作实验时间,同时也减少吸取液体次数,进而降低操作所产生的误差。 本PCR预混试剂盒含有五倍立即使用预混液并附赠六倍DNA上样指示染剂(蓝),其中五倍即用型混合液包含Taq DNA聚合酶、反应缓冲液、氯化镁、dNTsP和酶稳定剂;而DNA上样指示染剂 (蓝)含有二种追踪染剂 (Xylene cyanol FF 与 Bromophenol blue)可用于观察DNA片段在琼脂醣凝胶中的大略位置。The ExcelTaq 5× PCR Master Mix is a ready-to-use mixture for amplifying targeted DNA fragments. It is designed to serve as ready-to-use master mix for virtually all PCR applications. The mixture contains all essential ingredients for PCR with the exception of template and primers. This not only saves valuable time in the laboratory, but also reduces the number of pipetting and reagent handling errors. The PCR Master Mix is supplied as a 5× concentrated master mix, that is a mixture of recombinant Taq DNA polymerase, reaction buffer, MgCl2, 200 μM dNTPs, enzyme stabilizer.