详细描述:
ExcelTaq高保真性Klen-Taq DNA聚合酶(ExcelTaq Klen-Taq Polymerase, TK1000)以更适当比例混合了KlenTaq-1 DNA 聚合酶(切除N端,缺少5'→3' 外切酶活性的DNA 聚合酶)及少量具有校正活性的DNA聚合酶。 此一独特的设计可以提高保真性、延伸性及扩增效率。 Klen-Taq DNA聚合酶可以耐受不同浓度的镁离子,它非常耐热且保真性是一般Taq DNA聚合酶的4倍。 TK1000高保真性Klen-Taq DNA聚合酶非常适合从基因组DNA扩增0.5-5 kb的目标片段,在处理较单纯模版的情况则可以到达10 kb。ExcelTaq Klen-Taq DNA Polymerase is a specially blended enzyme mix containing KlenTaq-1 DNA polymerase (a 5’-exo-minus, N-terminal deletion of Taq DNA polymerase) and a small amount of a proofreading DNA polymerase. This unique blending helps to improve the fidelity, yield and processivity of the resultant PCR process. The Klen-Taq is also highly robust, showing high tolerance of varying concentration of Mg2+; it is highly thermostable and has four times the fidelity compared to Taq DNA polymerase. The ExcelTaq Klen-Taq DNA Polymerase is ideal for DNA amplifications 0.5-5 kb in length on genomic DNA, and up to 10 kb on less complex templates.