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Stellaris原位杂交探针,Quasar® 670标记的小鼠Gapdh探针

Stellaris® FISH Probes, Mouse Gapdh with Quasar® 670 Dye

产品概述 技术指标 配套产品 订购信息 精彩视频 相关资料
小鼠Gapdh探针包括一组Quasar® 670标记的寡核苷酸以相同的比例混合在一起,终浓度是1 nmol,按照标准条件操作能够做大概80次杂交。该探针用于利用荧光原位杂交的方法检测小鼠Gapdh的转录本。 探针设计依据:小鼠甘油醛-3-磷酸脱氢酶(Mouse glyceraldehyde-3-phosphate dehydrogenase, Gapdh), 又名G3PD、Gapd (NCBI gene ID: 14433), 针对编码序列NM_008084.2 第51-1052段序列设计。 该探针没有在同物种或其它物种的同源和直系同源序列上验证可能的交叉杂交。用Quasar 670染料标记的探针组检测小鼠Gapdh mRNAs的代表图片是在NIH 3T3细胞上完成的。 Mouse Gapdh consists of a set of Quasar® 670-labeled oligos mixed at equal ratios and pooled into a final delivered amount of 1 nmol, which yields approximately 80 hybridizations under standard conditions. Designed to detect Gapdh transcripts in Mouse specimens using fluorescence in situ hybridization (FISH). Design Criteria: Product was designed against Mouse glyceraldehyde-3-phosphate dehydrogenase, Gapdh, a.k.a. G3PD; Gapd; (NCBI gene ID: 14433), and the coding sequence of NM_008084.2 nts 51-1052.The probe set has not been tested for potential cross-hybridization to RNA(s) of paralogous and orthologous gene(s) in the same or other species. Representative image of mouse Gapdh mRNAs detected with a Quasar 670 dye labeled probe set in NIH 3T3 cells.
属性: 更大激发荧光(Lambda Max):647; 更大发射荧光:670; 产品用法: 溶解后的探针注意尽量避免反复冻融。当天或短期使用,探针溶液可以避光放在+2 ~ +8 °C储存一个月,超过1个月,推荐分装后将溶解后的探针放在-15至-30 °C避光保存。 Protocols:详细Protocol根据样本类型而异,请上以下网站下载:https://www.biosearchtech.com/stellarisprotocols; 储存及运输: 运输条件:常温/蓝冰/干冰; 储存条件:+2 ~ +8 °C; Properties: Product Usage: Absorption Maximum (Lambda Max): 647; Fluorescence Maximum: 670; Product Usage: Please note that reconstituted probe mix should be subjected to a minimum number of freeze-thaw cycles. For daily and short-term use, the mix can be stored at +2 to +8 °C in the dark for up to a month. For long-term use: We recommend freezing reconstituted probes in the dark at -15 to -30 °C for storage lasting longer than a month. Protocols: Detailed protocols by sample type can be found here: https://www.biosearchtech.com/stellarisprotocols. Storage and Handling: Shipping Conditions: Ambient; Storage Conditions: +2 to +8 °C
货号 产品名称 单位 购买
SMF-HB1-10 Stellaris RNA FISH杂交缓冲液 10 mL 商城订购
SMF-WA1-60 Stellaris RNA FISH洗涤缓冲液A 60 mL 商城订购
SMF-WB1-20 Stellaris RNA FISH洗涤缓冲液B 20 mL 商城订购
货号 名称 单位 购买
SMF-3140-1 Stellaris原位杂交探针,Quasar® 670标记的小鼠Gapdh探针 1 nmol 商城订购
VSMF-3015-5 Stellaris原位杂交探针,Quasar® 670标记的小鼠Gapdh探针 5 nmol 商城订购

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