详细描述:
ExcelDye六倍DNA 即用型上样指示染剂(ExcelDye 6X DNA Loading Dye, DL4000)适用于DNA 琼脂醣凝胶或聚丙烯酰胺电泳分析,帮助DNA上样并观测电泳过程。 本产品含有二甲苯腈蓝FF (Xylene cyanol FF)、溴酚蓝(Bromophenol blue)及橙黄G (Orange G)可用于追踪电泳过程。 它们在 2% TAE的琼脂醣凝胶的大约位置相当于800 bp、150 bp与30 bp的DNA片段(或于1%的胶体约4000 bp、500 bp与50 bp大小)。 成分中含有甘油(glycerol)可使DNA上样时维持在胶体的底部,而EDTA成分则可螯合两价的金属离子,使需要金属的核酸酶失去活性。The ExcelDye 6× DNA Loading Dye (Tri-Color) is pre-mixed buffer for tracking the DNA sample during the electrophoresis on agarose or polyacrylamide gels. It contains three dyes (Xylene cyanol FF, Bromophenol blue, Orange G) for tracking the DNA migration. The Xylene cyanol FF, Bromophenol blue and Orange G migrate at approximately 800 bp, 150 bp and 30 bp on a standard 2% TAE agarose gel respectively (4,000 bp, 500 bp and 50 bp on 1% TAE agarose gel respectively). The included glycerol keeps the DNA at the bottom of the well and the presence of EDTA chelates divalent metal ions to prevent the process of metal-dependent nuclease.