化学发光蛋白marker（YesBlot™ Western Marker I，WM1000） 为即用型产品，内含10条IgG结合蛋白条带，片段范围涵盖15 kDa至200kDa。YesBlot™ Western Marker 可以有两重功能：一、包含4条预染蛋白条带，分别为10, 25, 45 and 70 kDa，用于只是电泳进程，估算蛋白大小及western印迹法的膜转印效率；二、10条IgG结合蛋白可以进行免疫杂交，在胶片或 CCD成像系统曝光。与化学发光，应该以及显色等检测系统兼容。另外，该Marker 在30 kDa and 80 kDa处做了增强处理，用于方便辨识条带大小。YesBlot™ Western Marker I is a ready-to-use mixture with ten IgG-binding proteins covering a wide range of molecular weights from 15 to 200 kDa in TrisGlycine buffer. YesBlot™ Western Marker I performs dual functions. First, it contains 4 pre-stained proteins (10, 25, 45 and 70 kDa) for monitoring protein separation during SDSPAGE, verification of Western transfer efficiency on membranes (nitrocellulose, PVDF, or nylon) and for approximating the protein size. Second, ten IgGbinding proteins can be immuno-detected on film or by CCD imaging. YesBlot™ Western Marker I is compatible for chemiluminescent, fluorescent, chromogenic or other detection systems. In addition, YesBlot™ Western Marker I has two reference bands with enhanced intensity (at 30 kDa and 80 kDa). The marker is supplied in the gel loading buffer and is ready to use. Do NOT heat, dilute, or add reducing agents before loading.
