详细描述:
ExcelTaq Q-PCR预混液(ExcelTaq Q-PCR Master Mix, TQ1100),包含PCR反应所有的必要成分,除引物与DNA模板需由用户自行添加。
本产品内含热启动 DNA polymerase及 与双链DNA专一结合的SYBR green荧光染料,且具有高扩增效率,进行高灵敏、高精确之扩增反应,实时监控追踪扩增步骤。 此产品Master Mix与常规性Real-Time PCR系统所需之ROX reference dye兼容使用。The ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) is a ready-to-use reagent with all the essential components for quantitative real-time PCR (qPCR) except primers and template. The master mix features high sensitivity and signal intensity as well as low background and better compatibility with cDNA templates derived directly from reverse transcription reaction mixture. The ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) contains hot-start Taq polymerase in an optimized buffer with dsDNA specific SYBR green fluorescent dye. This master mix allows for sensitive, precise amplification, real-time tracking of the amplification process, and simultaneous quantification for targeted DNA molecules. With inert smart blue contrast dye, the ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) is ready-to-use and greatly reduces pipetting errors, while largely improving the reproducibility of the process. The ExcelTaq™ 2X Q-PCR Master Mix is also compatible with a ROX reference dye (Cat. No. CF1000) if recommended by the manufacturer of the qPCR system