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KOD引物混合物

KASP On Demand Primer Mix

产品概述 技术指标 配套产品 订购信息 精彩视频 相关资料
KOD引物包含3条针对SNP或InDel特异性的KASP引物：两条等位基因特异性的正向引物和一条通用的反向引物，引物经过完整验证，能够保证100%有效。
For KASP on Demand (KOD) our scientists design and fully validate and optimise your assay prior to delivery. Any custom KASP assay can be provided with our validation service ensuring that you receive a working assay.

If you are working with human DNA samples, KOD assay validation can be performed using our validation panel of 44 DNA samples. We can use your own human DNA samples for validation if you prefer, and this is recommended if you are working on an assay with a low minor allele frequency. For all other organisms, LGC require the customer to send in sample DNA to enable assay validation to be carried out in our laboratories. We require a minimum of 22 DNA samples for validation, and will accept up to 94 DNA samples. For more information about how much DNA is required, please see our factsheet.

KASP assays have an industry-leading conversion rate. We have carried out the design for millions of individual assays and we have significant experience in the design of successful assays for even the most difficult genomes or target sequences. With our validation service we guarantee delivery of a working assay or you will not be charged.
订购需填写定制表格。
KASP Primer Mix存放于单个带有二维码的试剂管中，便于管理与追踪：
用96孔PCR板，10 uL体系，可以检测2500次反应；
用384孔PCR板，5 uL体系，可以检测5000次反应。
标准服务的货期是4-6周，快速服务的货期是2-3周。

Ordering
1. Please request a quotation or enquire about pricing.
2. We will email you with the quotation for your assays, and will include a sequence submission form
3. Review your quotation and if you’re happy with it, submit a purchase order to the address provided.
4. Next, complete and return the sequence submission form with your sequence/s of interest and polymorphism/s market to tell us which SNPs / InDels you are interested in
5. Assay design (primer selection) will then be carried out using our proprietary Kraken™ software system, or manually by our scientists where required.
6. Our scientists fully validate and optimise your assay(s). For all species, with the exception of human, we require a minimum of 22 DNA samples (maximum 94 DNA samples) to enable the assay to be validated in our laboratories. Please complete a DNA submission form to identify your samples.
7. All three target-specific primers for an assay are combined into a single 2D barcoded tube
8. Each KOD Assay mix is shipped in a 2D barcoded tube, with an additional printed label.

Delivery
Aliquots of KOD Assay mix are provided in 2D barcoded tubes in sufficient quantities to enable
2,500 reactions at 10 µL total reaction volumes (96-well PCR plates)
5,000 reactions at 5 µL total reaction volumes (384-well PCR plates).

Our Standard service provides an estimated delivery of 4-6 weeks.
Our Fast delivery service will guarantee delivery of the functionally validated KASP assays in 2-3 weeks.
货号 产品名称 单位 购买
KBS-1016-001 KASP v4.0 2X预混液，标准ROX（96/384孔板） 2.5mL 商城订购
KBS-1016-002 KASP v4.0 2X预混液，标准ROX（96/384孔板） 25mL 商城订购
KBS-1016-016 KASP v4.0 2X预混液，低ROX 2.5mL 商城订购
KBS-1016-017 KASP v4.0 2X预混液，低ROX 25mL 商城订购
KBS-1016-021 KASP v4.0 2X预混液，高ROX 2.5mL 商城订购
KBS-1016-022 KASP v4.0 2X预混液，高ROX 25mL 商城订购
货号 名称 单位 购买
KBS-2000-100 KOD引物混合物 2,500x10ul reactions, std service 商城订购
KBS-2000-101 KOD引物混合物 2,500 x 10 uL reactions 商城订购
KBS-2000-200 KOD引物混合物 2,500x10ul reactions, std service Manual Desi 商城订购
KBS-2000-300 KOD引物混合物 2,500x10ul reactions, std service Manual Desi 商城订购
KBS-2000-400 KOD引物混合物 2,500x10ul reactions, std service Manual Desi 商城订购
KBS-2000-500 KOD引物混合物 2,500x10ul reactions, std service, Additional 商城订购
KBS-2000-150 KOD引物混合物 2,500x10ul reactions, rapid service 商城订购
KBS-2000-250 KOD引物混合物 2,500x10ul reactions, rapid service Manual De 商城订购
KBS-2000-350 KOD引物混合物 2,500x10ul reactions, rapid service Manual De 商城订购
KBS-2000-450 KOD引物混合物 rapid service Manual Design (plus Pre-Amp Pri 商城订购
KBS-2000-550 KOD引物混合物 2,500x10ul reactions, rapid service, Addition 商城订购
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