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SuperPure™纯化柱(<= 50 nmol合成规模)

SuperPure™ Purification Column (<= 50 nmol s

产品概述 技术指标 配套产品 订购信息 精彩视频 相关资料
Oligonucleotides (whether synthetic or natural) may be purified by a number of techniques, including polyacrylamide gel electrophoresis and high performance liquid chromatography (either by ion exchange or reverse-phase methods). Synthetic DNA can be conveniently de-salted and purified by reverse-phase low-pressure separation on BTI's SuperPure (SP-1000) columns. BTI's SuperPure Columns are intended for reverse-phase purification of 5'-DMT oligonucleotides followed by on-column detritylation. The method relies on strong binding between the 5'-DMT protected product and a hydrophobic optimized polymer packing (polystyrene), thus allowing separation from truncated sequences which, due to a lack of DMT group, do not bind. Treatment with acid removes the DMT group from the product, the desired product (free from organic residues) is eluted with 20% acetonitrile. The method is rapid, efficient and permits many samples to be purified simultaneously. The SuperPure column has capacity to handle crude, cleaved DNA from a 50 nmol scale synthesis. The purified yield will depend, in each case, on sequence length and the quality of the crude sample.
https://www.biosearchtech.com/products/synthesis-reagents/synthesis-and-purification-accessories/superpure8482-purification-column-50-nmol-scale-synthesis
货号 产品名称 单位 购买
CL-1501-10 DNA合成柱 大号空柱，10包 商城订购
FR-1501-100 筛板，柱子 1/16英寸 x 0.163, 100 Pack 商城订购
BG5-5081-1 CAL Fluor Orange 560 CPG (5'-DMT-T(C6-CAL Flu 1 g 商城订购
货号 名称 单位 购买
SP-1000-1 SuperPure™纯化柱(<= 50 nmol合成规模) 1 商城订购
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