Phospho-Threonine-X-Arginine Antibody识别内源性的包含磷酸化Thr-X-Arg基序的蛋白。此抗体识别+2位是精氨酸或赖氨酸的磷酸化苏氨酸，对+2位是赖氨酸比+2位是精氨酸的识别能力稍弱。此抗体与包含非磷酸化苏氨酸和非磷酸化丝氨酸的相同基序没有交叉反应。其识别PKCβ中FFT*R模体里的磷酸化苏氨酸，但不识别其它缺乏+2位赖氨酸或精氨酸模体中的磷酸化苏氨酸。（美国号：6,441,140、6,982,318、7,259,022、7,344,714；U.S.S.N. 11,484,485；及所有国外相应）.该多克隆抗体用合成的含磷酸化Thr-X-Arg基序的肽段免疫动物制备。该抗体使用蛋白A和蛋白亲和层析纯化。Phospho-Threonine-X-Arginine Antibody detects endogenous levels of proteins containing the phospho-Thr-X-Arg motif. This antibody detects phosphorylated Thr followed by Arg or Lys at the +2 position, though its reactivity is lower for Lys compared to Arg at the +2 position. The antibody does not cross-react with nonphospho-Thr or phospho-Ser in the same motif. It recognizes phospho-Thr in the FFT*R motif in PKCbeta II but does not recognize phospho-Thr in other motifs that lack Lys or Arg at +2. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide containing the phospho-Thr-X-Arg motif. Antibodies are purified by protein A and peptide affinity chromatography.
应用范围：W IHC-P E-P； 反应种属：Human,Mouse,Rat,Monkey,All Species Expected； 灵敏度：Endogenous；Isotype：Rabbit； 标记：无标记。
