该抗体检测仅在serine 27位点磷酸化的内源性SirT1蛋白。该抗体不能与其它sirtuin蛋白发生交叉反应。通过合成的与人源SirT1蛋白Ser27位点周围相应的磷酸化片段去免疫动物从而制备出多克隆抗体。通过蛋白A和多肽亲和层析纯化获得。This antibody detects endogenous levels of SirT1 protein only when phosphoryated on serine 27. The antibody does not cross-react with other sirtuin proteins. Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser27 of human SirT1. Antibodies are purified by protein A and peptide affinity chromatography.