Phospho-(Ser) PKC Substrate Antibody可识别内源性在+2和-2位上有精氨酸或赖氨酸，并且+1位是疏水氨基酸的磷酸化丝氨酸的细胞内蛋白质。该抗体不与非磷酸化丝氨酸残基、同样基序中磷酸化的苏氨酸残基、或其它基序中的磷酸化丝氨酸残基发生交叉反应。（美国号：6,441,140、6,982,318、7,259,022、7,344,714；U.S.S.N. 11,484,485；及相应国外）该多克隆抗体用合成的磷酸化PKC底物肽段免疫动物制备。该抗体使用蛋白A和多肽亲和层析纯化。Phospho-(Ser) PKC Substrate Antibody detects endogenous levels of many cellular proteins only when phosphorylated at serine residues surrounded by Arg or Lys at the -2 and +2 positions and a hydrophobic residue at the +1 position. The antibody does not cross-react with nonphosphorylated serine residues, with phospho-threonine in the same motif, or with phospho-serine in other motifs. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)Polyclonal antibodies are produced by immunizing animals with synthetic phospho-PKC substrate peptides . Antibodies are purified by protein A and peptide affinity chromatography.
