磷酸化HER2/ErbB2 (Tyr1248)/ EGFR (Tyr 1173) 抗体仅在Tyr1248被磷酸化时才能检测到ErbB2的存在,仅在Tyr1173被磷酸化时才能检测到EGFR的存在。多克隆抗体通过用多肽免疫动物得到,该磷酸化多肽是根据人的ErbB2蛋白Tyr1248附近的氨基酸序列合成的。抗体经过protein A和亲和层析纯化。Phospho-HER2/ErbB2 (Tyr1248)/ EGFR (Tyr 1173) Antibody detects ErbB2 only when phosphorylated at tyrosine 1248 and EGFR only when phosphorylated at tyrosine 1173.Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr1248 of human ErbB2. Antibodies are purified by protein A and peptide affinity chromatography.