磷酸化的EGFR (Tyr845)的抗体仅在Tyr845被磷酸化后才能检测到内源EGFR的水平。本抗体可能与EGFR家族的其它蛋白发生交叉反应(如ErbB2)。多克隆抗体通过用多肽免疫动物得到,该磷酸化多肽是根据人的EGFR蛋白Tyr845附近的氨基酸序列合成的。抗体经过protein A和亲和层析纯化。Phospho-EGF Receptor (Tyr845) Antibody detects endogenous levels of EGF receptor only when phosphorylated at tyrosine 845. The antibody may cross-react with other activated EGF receptor family members (e.g., ErbB2).Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr845 of human EGF receptor. Antibodies are purified by protein A and peptide affinity chromatography.