详细描述:
此抗体套装包含有PGAM1 (D3J9T) Rabbit mAb #12098T、PFKFB2 (D7G5R) Rabbit mAb #13045T、PFKFB3 (D7H4Q) Rabbit mAb #13123T、Enolase-1 Antibody #3810T、PDHK1 (C47H1) Rabbit mAb #3820T、Anti-rabbit IgG, HRP-linked Antibody #7074P2、Aldolase A (D73H4) Rabbit mAb #8060T、Enolase-2 (D20H2) Rabbit mAb #8171T和PFKL Antibody #8175T。Glycolysis II Antibody Sampler 试剂盒提供了一种经济的方式研究参与糖酵解的一些酶。该试剂盒提供了足以进行4次western blot分析的一抗。分别合成与人果糖二磷酸醛缩酶A的Pro263邻近氨基酸残基序列,人烯醇化酶2蛋白的羧基端序列,人PFKFB2蛋白果糖2,6 -二磷酸酶区域的Pro454邻近氨基酸残基序列,人PFKFB3蛋白的Leu456邻近氨基酸残基序列,和人PGAM1羧基端序列一致的肽段免疫动物获得单抗。分别合成与人烯醇化酶1和人PFKL蛋白羧基端序列一致的肽段免疫动物获得多抗。多抗由蛋白A和肽亲和层析纯化。The Glycolysis II Antibody Sampler Kit provides an economical means to investigate select enzymes involved in glycolysis. The kit contains enough primary antibody to perform four western blot experiments per primary antibody.Each antibody recognizes endogenous total levels of the specified target protein independent of its modified state.Monoclonal antibodies are produced by immunizing animals with a synthetic peptides corresponding to residues surrounding Pro263 of human fructose bisphosphate aldolase A protein, the carboxy terminus of human enolase-2 protein, human PDHK1 protein, residues surrounding Pro454 within the fructose-2,6-biphosphatase region of human PFKFB2 protein, residues surrounding Leu456 of human PFKFB3 protein, and the carboxy terminus of human PGAM1 protein. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human enolase-1 and carboxy terminus of human PFKL protein. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.