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流式检测试剂盒套装
Human T/B/NK Flow Kit(CD3 FITC/CD16+56 PE/CD45 PerCP/CD19 APC)
| DESCRIPTION | DESCRIPTION: |
| STORAGE | STORAGE: |
| Materials Provided | Materials Provided: |
| Related Protocol | Related Protocol： |
背景信息:
T regulatory (Treg) cells are a subset of T lymphocytes which is characterized by CD4+/CD25+/CD127-. These naturally occurring Treg cells originate in the thymus, and comprise 2-10% of peripheral CD4+ T cells. It has been shown that Treg cells are able to inhibit T cells proliferation and cytokine production and play critical roles in preventing autoimmunity as well as in controlling tumor immunity and transplantation tolerance. Impaired Treg function or Treg cell deficiency will develop variety of autoimmune diseases, while higher frequency of Treg cells will cause hypo-immune response to pathogens.
Total T lymphocyte percentages can be used to characterize some forms of immunodeficiency and autoimmune diseases. Determining percentages of CD4+ and CD8+ lymphocytes can be useful in monitoring the immune status of patients with immune deficiency diseases, autoimmune diseases, or immune reactions. The relative percentage of the CD4+ subset has been reported to be depressed and the relative percentage of the CD8+ subset to be elevated
in many patients .
Helper/inducer T lymphocytes are a subset of T (CD3+) lymphocytes that also express the CD4 antigen. Suppressor/cytotoxic T lymphocytes express the CD8 antigen and are principally a subset of T (CD3+) lymphocytes.
The CD4+/CD8+ lymphocyte ratio can be quantified as the ratio of CD4 FITC–positive lymphocytes to CD8 PE–positive lymphocytes. The most common method that has been used to determine the CD4/CD8 ratio has been a single two-color reagent containing CD4 and CD8 antibodies. The CD4+/CD8+ ratio has been used to evaluate the
immune status of patients with autoimmune disorders or those suspected of developing immune disorders.
The conventional helper/suppressor ratio (CD4/CD8) does not distinguish between T- and NK-lymphocyte CD8 antigen expression, or between helper/inducer T lymphocytes and monocytes that express the CD4 antigen in low copy numbers. The reagent combinations provided in the kit allow the CD3+CD4+ helper/inducer T lymphocytes and CD3+CD8+ suppressor/cytotoxic T lymphocytes to be identified and enumerated separately from contaminating CD3– CD4+ monocytes and CD3–CD8+ NK lymphocytes.22 Instead of using a single reagent containing CD4 and CD8.
NK lymphocytes, identified as being CD3–, CD16+ and/or CD56+ have been shown to mediate cytotoxicity against certain tumors and virus-infected target cells.
CD19 is involved in B cell development.B Cells which is characterized by CD3–, CD19+ .
| 货号 | 产品名称 | 单位 | 购买 |
|---|
| 货号 | 名称 | 单位 | 购买 |
|---|---|---|---|
| FHM003KI-G025 | 流式检测试剂盒套装 | 25 Tests | 商城订购 |
| FHM003KI-G050 | 流式检测试剂盒套装 | 50 Tests | 商城订购 |
| FHM003KI-G100 | 流式检测试剂盒套装 | 100 Tests | 商城订购 |
| FHM004KI-G025 | 流式检测试剂盒套装 | 25 Tests | 商城订购 |
| FHM004KI-G050 | 流式检测试剂盒套装 | 50 Tests | 商城订购 |
| FHM004KI-G100 | 流式检测试剂盒套装 | 100 Tests | 商城订购 |
