| DESCRIPTION | DESCRIPTION: |
| STORAGE | STORAGE: |
| Materials Provided | Materials Provided: |
| Related Protocol | Related Protocol： |
背景信息:
The peripheral CD4+ T cell pool includes multiple effector and memory T cell subsets that arise through antigendriven expansion and differentiation of naïve T cells. The early response of naïve CD4+ T cells to antigenic stimulation
is characterized by high level proliferation and a limited cytokine repertoire. Further differentiation yields cells with a
more diverse potential for cytokine expression. Depending upon the balance of local cytokines, costimulatory
molecules, antigen levels, and genetic factors, Type-1 T helper (Th1), Th2 effector and/or memory cells are generated
by immune responses.
Functionally-polarized CD4+ T cell subsets have been identified based on their distinctive patterns of cytokine
secretion. As a signature cytokine, Th1 cells selectively produce large amounts of interferon-gamma (IFN-γ). Th2 cells
selectively produce IL-4,. Through secretion of IFN-γ and other effector molecules, Th1 cells activate macrophages,
natural killer (NK) cells, and CD8+ T cells and are responsible for cell-mediated immunity. Th1 cells provide protection
against intracellular bacteria, fungi, protozoa and viruses and are involved in some autoimmune responses. IL-4
produced by Th2 cells is particularly strong in driving B cells to generate IgE-secreting cells. IgE plays a role in
basophil/mast cell mediated immune reactions. Th4 cells mediate protection against extracellular parasites but may
also cause harmful allergic responsiveness to develop.
The kit can be used to successfully analyze ex vivo lymphoid cell samples (eg, for the types of in vivo-generated
human peripheral blood T helper cells) or to monitor T helper cell differentiation by cells cultured within various
experimental model systems
