详细描述:
4-羟基-3-硝基苯基乙酸活性酯可用于与蛋白质偶联以形成能产生抗体的具免疫原性的全抗原。
4-Hydroxy-3-nitrophenylacetic acid active ester is useful for conjugating to proteins in order to form immunogenic agents used to raise antibodies.
Properties:
Chemical Name: 4-Hydroxy-3-nitrophenylacetic acid Succinimide Ester;
Formula: C12H10N2O7;
Molecular Weight: 294.22;
Appearance: pale yellow powder;
Absorption Maximum (Lambda Max): 430;
Extinction Coefficient at Lambda max: 4230;
Product Usage:
For conjugation to protein or DNA, dissolve in DMSO. NP OSu Conjugation to a protein: Add NP OSu to protein pre-dissolved in HCO3- buffer (pH ca 8.0) (Protein Concentration: Approx. 10 mg/mL). Stir 1 day at room temperature. Spin down insoluble material. Decant soluble fraction and dialyze until no color escapes (2-3 days). Lyophilize. Determine substitution ratio @430 nm, pH 8.5 Take a spectral scan of the solution using a spectrophotometer. Absorbance should be less than 1.0. Use the absorbance value at 430 nm to determine the loading of NP per unit of protein.
Storage and Handling:
Shipping Conditions: Ambient;
Storage Conditions: -15 to -30 °C