详细描述：
此抗体套装包含IRF-3 (D6I4C) XP® Rabbit mAb #11904、IKKε (D20G4) Rabbit mAb #2905、TBK1/NAK (D1B4) Rabbit mAb #3504、Rig-I (D14G6) Rabbit mAb #3743、MAVS Antibody #3993、Phospho-IRF-3 (Ser396) (4D4G) Rabbit mAb #4947、MDA-5 (D74E4) Rabbit mAb #5321、Phospho-TBK1/NAK (Ser172) (D52C2) XP® Rabbit mAb #5483、Phospho-IKKε (Ser172) (D1B7) Rabbit mAb #8766各20µl，以及含Anti-rabbit IgG, HRP-linked Antibody #7074 100µl。Rig-I Pathway Antibody Sampler Kit为研究Rig-I 信号通路上多个成员的活性状态和总蛋白水平提供了经济的方法，包括Rig-I, MDA-5, MAVS, IRF-3, TBK1/NAK和IKKε。本试剂盒内为每个抗体提供了足够四次Western blots 所需的一抗。MDA-5 (D74E4) Rabbit mAb, Rig-I (D14G6) Rabbit mAb, MAVS Antibody, IRF-3 (D83B9) Rabbit mAb, TBK1/NAK (D1B4) Rabbit mAb, IKKε (D61F9) XP® Rabbit mAb 和 IKKε (D20G4) Rabbit mAb 分别能够检测对应的内源水平总蛋白，并且不与其它的蛋白交叉反应。MAVS Antibody检测到 52 和75 kDa两条条带与Seth et al. (2005)描述的有关。Phospho-TBK1/NAK (Ser172) (D52C2) XP® Rabbit mAb 只能检测内源的在Ser172位点磷酸化的TBK1/NAK蛋白。Phospho-IRF-3 (Ser396) (4D4G) Rabbit mAb只能检测内源的在Ser396位点磷酸化的IRF-3蛋白。The Rig-I Pathway Antibody Sampler Kit provides an economical means to evaluate the activation state and total protein levels of multiple members of the Rig-I pathway including Rig-I, MDA-5, MAVS, IRF-3, TBK1/NAK, and IKKε. The kit includes enough primary antibody to perform four western blot experiments per antibody.MDA-5 (D74E4) Rabbit mAb, Rig-I (D14G6) Rabbit mAb, MAVS Antibody, IRF-3 (D83B9) Rabbit mAb, TBK1/NAK (D1B4) Rabbit mAb, IKKε (D61F9) XP® Rabbit mAb, and IKKε (D20G4) Rabbit mAb detect endogenous levels of respective total proteins and do not cross-react with other proteins. Bands detected at 52 and 75 kDa by MAVS Antibody correlate with those described by Seth et al. (2005). Phospho-TBK1/NAK (Ser172) (D52C2) XP® Rabbit mAb detects endogenous levels of TBK1/NAK only when phosphorylated at Ser172. Phospho-IRF-3 (Ser396) (4D4G) Rabbit mAb detects endogenous levels of IRF-3 only when phosphorylated at Ser396.
