详细描述:
此抗体套装包含Phospho-PLCγ1 (Tyr783) (D6M9S) Rabbit mAb #14008、Phospho-ALK (Tyr1586) (3B4) Rabbit mAb #3348、Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060、Phospho-Stat5 (Tyr694) (D47E7) XP® Rabbit mAb #4322、Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb #4370、Phospho-Jak2 (Tyr1007) (D15E2) Rabbit mAb #4406、Phospho-Jak3 (Tyr980/981) (D44E3) Rabbit mAb #5031、Phospho-Src Family (Tyr416) (D49G4) Rabbit mAb #6943、Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb #9145各20µl,以及含Anti-rabbit IgG, HRP-linked Antibody #7074 100µl。ALK Activation Antibody Sampler Kit为检测ALK通路上多种组分的活化状态提供了经济有效地手段,包括检测磷酸化的ALK, Jak2, Jak3, Stat3, Stat5, PLCγ1, Akt, Src和p44/42 MAPK。本试剂盒包含的抗体足以满足每种一抗进行4次免疫印迹检测。ALK Activation Antibody Sampler Kit中的每种抗体能检测其特定靶蛋白的磷酸化形式。磷酸化ALK (Tyr1586) (3B4)兔单抗可能与包括EGFR在内的激活的酪氨酸激酶蛋白交叉反应。磷酸化Jak2 (Tyr1007) (D15E2) 兔单抗可能与磷酸化的Jak3 和Tyk2发生交叉反应。磷酸化Src 家族(Tyr416) (D49G4) 兔单抗可能与过表达的磷酸化的RTKs发生交叉反应。The ALK Activation Antibody Sampler Kit provides an economical means to evaluate the activation status of multiple members of the ALK pathway, including phosphorylated ALK, Jak2, Jak3, Stat3, Stat5, PLCγ1, Akt, Src, and p44/42 MAPK. The kit includes enough antibody to perform four western blot experiments with each primary antibody.Each antibody in the ALK Activation Antibody Sampler Kit recognizes the phosphorylated form of its specific target. Phospho-ALK (Tyr1586) (3B4) Rabbit mAb may cross-react with other activated protein tyrosine kinases including EGFR. Phospho-Jak2 (Tyr1007) (D15E2) Rabbit mAb may cross react with phosphorylated Jak3 and Tyk2. Phospho-Src Family (Tyr416) (D49G4) Rabbit mAb may cross react with overexpressed phosphorylated RTKs.