详细描述:
此抗体套装包含Acetyl-NF-κB p65 (Lys310) (D2S3J) Rabbit mAb #12629、Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb #3033 、Phospho-NF-κB p65 (Ser468) Antibody #3039、NF-κB p65 (L8F6) Mouse mAb #6956、NF-κB p65 (D14E12) XP® Rabbit mAb #8242各20µl,和Anti-rabbit IgG, HRP-linked Antibody #7074、Anti-mouse IgG, HRP-linked Antibody #7076各100 µl。The NF-κB p65 Antibody Sampler Kit 包含了测试在Ser276和Ser536位点磷酸化的NF-κB p65/RelA ; Lys310位点乙酰基化的p65/RelA ; 总p65蛋白水平。总NF-κB p65抗体能够检测内源水平p65蛋白而不与其翻译后的修饰状态如磷酸化或乙酰化无关。phospho-NF-κB p65抗体识别内源水平在特定位点磷酸化的p65蛋白。当磷酸化发生在同源位点时,phospho-NF-κB p65 (Ser276)抗体也能与c-Rel 和 NF-κB1 p105/p50抗体交叉反应(分别是Ser267和Ser337)。acetyl-NF-κB p65 (Lys310)抗体识别转染水平的在 Lys310位点乙酰化的p65蛋白。The NF-κB p65 Antibody Sampler Kit contains reagents to examine NF-κB p65/RelA phosphorylation at Ser276 and Ser536; acetylation at Lys310; and total p65 levels.The total NF-κB p65 antibodies recognize endogenous levels of p65 regardless of post-translational modification state such as phosphorylation or acetylation. The phospho-NF-κB p65 antibodies recognize endogenous levels of p65 only when phosphorylated at their indicated target residues. The phospho-NF-κB p65 (Ser276) antibody may also cross-react with c-Rel and NF-κB1 p105/p50 when phosphorylated at the homologous residues (Ser267 and Ser337, respectively). The acetyl-NF-κB p65 (Lys310) antibody recognizes transfected levels of p65 only when acetylated at Lys310