详细描述:
此抗体套装包含LC3A/B (D3U4C) XP® Rabbit mAb #12741T、Atg5 (D5F5U) Rabbit mAb #12994T、Atg3 Antibody #3415T、Beclin-1 (D40C5) Rabbit mAb #3495T 、Atg12 (D88H11) Rabbit mAb #4180T、Anti-rabbit IgG, HRP-linked Antibody #7074P2、Atg16L1 (D6D5) Rabbit mAb #8089T和Atg7 (D12B11) Rabbit mAb #8558T。Autophagy Antibody Sampler 试剂盒提供了一种经济有效的方法,研究细胞中自噬的分子机制。这种试剂盒包含了足够的一抗和二抗,可进行4次Western blot试验。Autophagy Antibody Sampler 试剂盒中的每个抗体都能够检测内源性的目的蛋白。Atg5和Atg12兔单抗能够识别Atg12-Atg5的复合物。LC3A和LC3B 兔单抗可以与其它形式的LC3发生交叉反应。在进行Western blot实验时,LC3B兔单抗对于II型的LC3B具有更强的反应性。The Autophagy Antibody Sampler Kit provides an economical means to investigate the molecular machinery of autophagy within the cell. The kit contains enough primary and secondary antibodies to perform four Western blot experiments.Each antibody in the Autophagy Antibody Sampler Kit detects its respective target at endogenous levels. Both the Atg5 and Atg12 rabbit monoclonal antibodies recognize the Atg12-Atg5 conjugate. The LC3A and LC3B rabbit monoclonal antibodies may cross-react with other LC3 isoforms. The LC3B rabbit monoclonal antibody has stronger reactivity with the type II form of LC3B by western immunoblot.