详细描述:
此Smad 1/5/9蛋白及磷酸蛋白小包装抗体套装包含12534T*1、13820T*1、38454T*1、5753T*1、6944T*1、7074P2*1和9516T*1。Smad1/5/8 Antibody Sampler试剂盒提供了经济的方式检测BMP信号通路的靶蛋白。该试剂盒提供了足以进行四次western blot实验的一抗。针对磷酸化Smad蛋白的抗体仅能识别特定位点被磷酸化后的靶蛋白。所有的Smad2, Smad3, 和 Smad4抗体都可以识别其各自内源性的靶蛋白。合成对应人Smad1 Ser206和人Smad5 Ser463/465邻近氨基酸残基序列一致的磷酸多肽,免疫动物获得磷酸化特异性单克隆抗体。Smad1 和 Smad5总蛋白单克隆抗体通过使用对应小鼠Smad1的Ser190和Smad5的Pro249邻近氨基酸残基序列的多肽,免疫动物获得单克隆抗体。使用对应人Smad5 Ser463/465和Smad4 Pro278及其邻近氨基酸残基序列的合成多肽免疫动物获得多抗。抗体由蛋白A和肽段亲和层析技术纯化得到。The Smad1/5/8 Antibody Sampler Kit provides an economical means of detecting target proteins of the BMP signaling pathway. The kit contains enough primary antibodies to perform four western blots with each.Activation state antibodies detect their intended targets only when phosphorylated at the indicated site. The total Smad1, Smad4, and Smad5 antibodies detect their respective targets at endogenous levels.Phospho-specific monoclonal antibodies are produced by immunizing animals with synthetic phosphopeptides corresponding to residues surrounding Ser206 of human Smad1 protein or Ser463/465 of human Smad5. Total Smad1 and Smad5 monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Ser190 of human Smad1 and Pro249 of human Smad5 protein. Polyclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to the residues surrounding Ser463/465 of human Smad5 or Pro278 of human Smad4. Antibodies are purified by protein A and peptide affinity chromatography.