详细描述:
此抗体组合kit包含2435T*1、2789T*1、3180T*1、3544T*1、3676T*1、7074P2*1、8178T*1、9349T*1。Adipogenesis Marker Antibody Sampler试剂盒提供了一种经济的方式检测参与调控脂肪形成的蛋白。该试剂盒提供了足以进行4次western blot分析的一抗。每个抗体都可以识别其各自内源性的靶蛋白。Adiponectin (C45B10) Rabbit mAb兔单抗可以识别内源性智联素总蛋白单体水平。它不会检测到大分子量的脂联素。Acetyl-CoA Carboxylase (C83B10) Rabbit mAb兔单抗可以识别内源性所有亚型的acetyl-CoA carboxylase总蛋白水平。单抗由合成肽段免疫动物获得,抗体分别和human acetyl-CoA carboxylase α1的Ser523,人脂联素,人FABP4的序列,以及人脂肪酸合成酶的Gly46邻近序列,人脂周素蛋白Ile419邻近序列,人PPARγ的Asp69邻近序列一致。合成与人C/EBPα的序列一致的肽段免疫动物获得单克隆抗体。多抗使用蛋白A和肽段亲和层析纯化。The Adipogenesis Marker Antibody Sampler Kit provides an economical means to evaluate proteins involved in the regulation of adipogenesis. The kit includes enough antibody to perform four western blot experiments with each primary antibody.Each antibody recognizes endogenous total levels of its specific target protein. The Adiponectin (C45B10) Rabbit mAb detects endogenous levels of total adiponectin protein monomer. It will not detect higher molecular weight forms of adiponectin. The Acetyl-CoA Carboxylase (C83B10) Rabbit mAb detects endogenous levels of all isoforms of acetyl-CoA carboxylase protein.Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser523 of human acetyl-CoA carboxylase α1, to human adiponectin, to the sequence of human FABP4, to residues surrounding Gly46 of human fatty acid synthase, to residues surrounding Ile419 of human perilipin protein, or to residues surrounding Asp69 of human PPARγ. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human C/EBPα. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.