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微球菌核酸酶
Micrococcal Nuclease
Micrococcal nuclease is a relatively non-specific endo-exonuclease derived from Staphylococcus aureus. Active in the pH range of 7.0-10.0, this product digests double-stranded, single-stranded, circular, and linear nucleic acids. In the SimpleChIP® Enzymatic Chromatin IP kit assay, following cell lysis, the chromatin is fragmented by partial digestion with micrococcal nuclease to obtain chromatin fragments of 1 to 5 nucleosomes in size. Enzymatic digestion of chromatin is much milder than sonication and eliminates problems due to variability in sonication power and emulsification of chromatin during sonication, which can result in incomplete fragmentation of chromatin or loss of antibody epitopes due to protein denaturation and degradation. This product is offered to conveniently provide additional micrococcal nuclease when fragmenting chromatin with our SimpleChIP® (#9002, #9003) and SimpleChIP® Plus (#9004, #9005) Enzymatic Chromatin IP Kits. These kits provide all the reagents required for performing 6 chromatin preparations (or optimizations) and 30 chromatin immunoprecipitation (ChIP) assays, however there are instances where extra micrococcal nuclease is desired.
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